#7 Resveratrol supplementation improves maternal glucose tolerance and prevents gestational diabetes-induced cardiometabolic disease development in the rat offspring
Gabriel M Brawerman, University of Manitoba/CHRIM; Stephanie M Kereliuk, University of Manitoba/CHRIM; Troy J Pereira, University of Manitoba/CHRIM; Bo Xiang, University of Manitoba/CHRIM; Mario A Fonseca, University of Manitoba/CHRIM; Vernon W Dolinsky, University of Manitoba/CHRIM
Gestational diabetes mellitus (GDM), which affects 5-10% of pregnancies, is characterized by hyperglycemia in the third trimester of pregnancy. GDM increases cardio-metabolic disease risk in the offspring. Resveratrol (RESV), a naturally produced polyphenol, has anti-oxidant properties and positive metabolic health effects. We hypothesize that RESV administration (150 mg/kg) to pregnant GDM dams in the third trimester will improve maternal glucose tolerance and protect offspring from GDM-induced obesity and heart disease.
Six weeks prior to mating, female Sprague-Dawley rats consumed a high fat and sucrose (HFS) diet (45% kcal fat) to induce GDM, while lean control females received a low fat (LF) diet (10% kcal fat). In the third trimester, a subgroup of pregnant HFS-fed rats were supplemented with RESV (150 mg/kg). After weaning, offspring were randomly assigned a HFS or LF diet for 12 weeks. Offspring lipid levels were analyzed by ELISA and dual energy X-ray absorptiometry scans were used to assess lean and fat body mass. Echocardiography was used to assess cardiac function and morphometry.
RESV improved maternal glucose tolerance, without affecting maternal body weight. In the neonatal offspring, RESV prevented GDM-induced elevated body and heart weights (p<0.05). GDM induced obesity in 15 week-old offspring and obesity was prevented in offspring exposed to GDM+RESV (p<0.05). Liver, cardiac, and circulating triglycerides were reduced in GDM+RESV offspring versus GDM offspring (p<0.05). Consumption of HFS by the offspring increased fat mass and percent body fat in all groups of offspring (p<0.05). GDM+RESV offspring exhibited similar heart weights to that of lean offspring but had reduced left ventricular posterior wall thickness against GDM offspring (p<0.05). Functional parameters were similar in all groups
Maternal RESV supplementation during the third trimester of pregnancy prevented GDM-induced obesity, cardiac hypertrophy, and cardiac and hepatic steatosis in the offspring.